Tilápia (tillapia rendali) comercializada na cidade de São Luís: avaliação da presença de coliformes e do perfil de sensibilidade antimicrobiana in vitro de escherichia coli

Abstract

Total coliforms are enterobacteria that ferment lactose, with gas production, when incubated at 35 ° C for 24 to 48 hours. In this group are mainly Escherichia, Klebsiella, Enterobacter and Citrobacter genera. The presence of Escherichia coli is a specific indicator of recent fecal contamination and the likely presence of pathogenic bacteria. Fecal coliforms are able to ferment lactose, with gas production in 24 to 48 hours at 45°C. The main representative of this group is the Escherichia coli, from faecal origin. This study aimed to evaluate the in vitro susceptibility of Escherichia coli isolated from tilapia (Tilapia rendalli) marketed in the city of São Luís - MA. The survey was conducted by the technique of multiple tube (most probable number) from decimal dilutions. For research of Escherichia coli we were performed smears stained by the Gram method and confirmed by biochemical tests. The in vitro susceptibility to antimicrobial was held by plates disk diffusion method in Muller Hinton agar. Between the means for coliforms at 35°C and coliforms at 45°C were found statistically significant differences. On fairs the NMP-/-g were higher than that found in markets. On the ten fair samples were found seven strains of Escherichia coli and on twenty supermarket samples, zero. One strain was resistant to antibiotic ampicillin, two showed intermediate resistance to antibiotics ampicillin and amoxicillin + clavulanic acid. It may result from use of antimicrobials in fish breeding.