Desenvolvimento de um protocolo de estabelecimento in vitro da bananeira

Abstract

Banana crop is of great importance as it is one of the most consumed fruits in the world and produced in large areas in Brazil. The state of Maranhão has regions considered as producing poles, thus requiring a great demand for seedlings with high phytosanitary quality to supply the need for commercial plantations. In this sense, tissue culture presents itself as an alternative for the production of seedlings with high phytosanitary and vegetative quality. The objective of this work was to develop an in vitro banana establishment protocol. For this, seedlings of the type horn, horn, horn and umbrella were collected from the banana plantation of the UEMA school farm, and in the laboratory were cleaned, reduced in size and disinfected. Aseptically in laminar flow they were cut to the apical meristem, disinfected again and established in three different culture media: MS medium, MS medium with addition of 2.5 μM BAP (6- benzylaminopurine) and MS medium with 0.5μM addition. ANA (Naphthalene Acetic Acid) and 1.5μM BAP (6-benzylaminopurine). Evaluations for explant response percentage were performed from the second to the fifth week after establishment. It was concluded that there was a low contamination rate in the experiment. The three culture media presented explant establishment at different response times, where the treatment with 2.5μM BAP showed faster