Pesquisa de mastite clínica e subclínica, contagem de células somáticas, susceptibilidade a antimicrobianos e resíduos de antibióticos em amostras de leite de búfalas (Babalus bubalis) da Baixada Maranhense

Abstract

The objective of this research work clinical and subclinical mastitis, somatic cell count, antimicrobial susceptibility and antibiotic residues in samples of buffalo milk. 304 buffaloes were evaluated 10 properties located in the municipalities of Viana, Arari, Cajari, Matinha and Victory Mearim , the low lands , using tests mug screened and California Mastiis Test (CMT). The 1204/4 mammary evaluated, only 1 (0.09 %) had clinical mastitis and 31 (2.57 %) had subclinical mastitis. We collected 31 samples positive for clinical and subclinical mastitis and microbiological analysis for the isolation and identification of micro -organisms. The residue antibiotics search was performed using commercial kits Delvotest SP-NT, Eclipse - Twinsensor 50 and BT-20. The antimicrobial susceptibility of strains isolated from milk was evaluated by disk diffusion. Positive samples, isolated Staphylococcus sp. (93.54 %), followed by Corynebacterium sp. (41.93 %), Enterobacteriaceae (38.70 %), Streptococcus sp. (32.25 %) and E. coli (32.25 %). In 1 (3.22 %) showed no microbial growth. Antimicrobials that showed greater efficacy for Staphylococcus coagulase positive were chloramphenicol and norfloxacin with 100 % sensitivity, and norfloxacin were coagulase negative Staphylococcus (91.6 %), gentamicin and norfloxacin with 86.1 %. In relation to Staphylococcus aureus, 100 % of the isolates were sensitive to novobiocin and 94.1 % to norfloxacin. The buffalo milk six properties in the low lands presented antimicrobial residue. The results indicate that preventive care are needed regarding the transmission of these pathogens through ingestion of contaminated milk and dairy products. Milk produced in low lands can pose risks to the population and the need to adopt measures to prevent and control mastitis based on the profile of antimicrobial susceptibility of micro - organisms causing disease.