Avaliação da atividade antitumoral da Carapa guianensis (Andiroba) sobre o tumor de Ehrlich sólido : ensaio in vivo

Abstract

Carapa guianensis Aublet, of the family Meliaceae, is a medicinal tree widely known and used by the communities of the region of the Amazon Forest. However, there are few studies related to Carapa guianensis and cancer treatment, requiring further research to help understand the antineoplastic potential of this plant. Thus, this work aims to evaluate the anti-tumor activity of Carapa guianensis in vivo in Swiss mice inoculated with neoplasic cells of Solid Ehrlich Tumor, through evaluations of pathological changes and COX-2 expression in the tumor mass. For the therapeutic trial, the animals were divided into seven groups: a control group, 3 groups treated with solutions of andiroba oil in different concentrations, and 3 groups treated with PBS and / or emulsifier (Tween-20) preparations. The animals were euthanized for removal of the primary lesion and organs, which were processed for histopathology and immunohistochemistry. Histological analysis revealed the presence of pathological alterations in the kidney, spleen and liver in animals of all the studied groups. Regarding the evaluation of the plantar cushion, all the treated groups showed some degree of necrosis, angiogenesis, invasiveness and inflammatory infiltrate. Therefore, under these experimental conditions, the solutions at different concentrations of Carapa guianensis oil did not demonstrate antineoplastic activity.