Identificação de Mycobacterium Avium Subsp. Paratuberculosis (MAP) através da bacterioscopia em búfalos abatidos em matadouros da Baixada Maranhense

Abstract

The Paratuberculosis is a granulomatous chronic enteritis wich is incurable and it is caused by the Mycobacterium avium subspecies paratuberculosis (MAP) which mainly affects the ruminant animals and might be pathogenic to another species of animals. It is considered responsible for economic losses that occur by decreased productivity and increased susceptibility to others infections, causing a negative impact on the economy. In this way, the aim of the present study is to diagnose the Mycobacterium avium subsp. paratuberculosis (MAP) in herds of slaughtered buffaloes of lowlands region in the state of Maranhao . It was collected tissue samples of small and large intestine, mesenteric lymphs and stool samples from 115 slaughtered buffalos from slaughterhouses of the Maranhao lowlands. For diagnosing that disease, histological examination with hematoxylin and eosin´s colouring (H&E), Ziehl-Neelsen and bacterial examination were made. It was possible to show granulomatous inflammatory infiltrate composed of epithelioid macrophages filled with Langhans giant cells in the mucosal mononuclear infiltrate in the submucosa, with atrophy and fusion of the villi in the small intestine, and severe diffuse infiltrate of epithelioid macrophages and Langhans giant cells in mesenteric lymph nodes, when subjected to histopathological diagnosis in 27% (31/115) of buffalo. With the Ziehl-Neelsen bacilli were visualized acid-alcohol resistant (AFB) in 23% (26/115) of samples from tissues analyzed. The stool samples were submitted to bacterial cultivation and it was viewed the Mycobacterium avium subsp. paratuberculosis at 19% (5/26). The results of this study show the subclinical occurring of Paratuberculosis in herds of slaughtered buffaloes in slaughterhouses of lowlands region in the state of Maranhão.