Identificação de Mycobacterium avium subsp. paratuberculosis através de reação de polimerase em cadeia (PCR) em búfalos abatidos em matadouros na Baixada Maranhense

Abstract

Paratuberculosis is a contagious disease, cosmopolitan, incurable that primarily affects domestic ruminants, causing a chronic granulomatous enteritis caused by the etiologic agent Mycobacterium avium subsp. paratuberculosis (MAP). It is responsible for reduced productivity and consequently large impact on the economy of buffalo of the Maranhão. This study aimed to identify Mycobacterium avium subsp. paratuberculosis (MAP) in buffalo slaughtered in slaughterhouses without inspection, in the Baixada Maranhense region and also characterize macroscopic lesions of mesenteric lymph node tissue, small and large intestine of buffalo with subclinical paratuberculosis. Samples of mesenteric lymph nodes were collected, small and large intestine of 115 buffalos being 66 females and 49 males. Macroscopic analysis of the fragments of organs was performed at the time of collection in accordance with: thickening of the intestinal mucosa and increased volume of mesenteric lymph node. Positive samples in bacterioscopy were submitted to qRT-PCR (quantitative real time PCR). From 26 positive samples in bacterial meningitis, 57.7% (15/26) were confirmed by molecular diagnosis. Based on the results it can be concluded that: a macroscopic evaluation identified lesions suggestive of subclinical paratuberculosis, which confirmed the presence of MAP in molecular diagnostics.