Reações inespecíficas em técnicas imunológicas para diagnóstico de anemia infecciosa equina e mormo

Abstract

Equine Infectious Anaemia and Glanders are the most important illnesses under the sanitary and economic point of view for herds of equines, according to the National Program of Sanity Equine (PNSE) since do not own treatment and effective vaccines. To control these illnesses, the Ministry of Agriculture, Livestock and Food Supply instituted compulsories sanitary measures in every national territory that include the official diagnosis by agar gel immunodiffusion test (AGIDT) for EIA and Complement Fixation (CF) for Glanders, and the sacrifice of the animals that are positive. To verify and to evaluate the occurrence of unspecific reactions by AGIDT and CF, it used 78 negative equine for EIA and Glanders, divided in groups and inoculated with different vaccines. After a week, the animals were submitted to five serology controls, repeated every seven days. The results indicated that the use of vaccines in horses before the official exams does not induce the seroconversion, since all the animals confirmed negative results. However, 11.11% of the horses vaccinated against influenza, encephalomyelites, equine rhinopneumonitis and tetanus, and 15.38% of those vaccinated against leptospirosis presented nonspecific negative reactions to AGIDT. Through of this study, the verification of nonspecific negative reactions in AGIDT for EIA's diagnosis it demonstrates a kind of result not boarded by the Portaria nº 84/1992 – SDA/MAPA and, therefore, it will be able to base alterations in the valid legislation about diagnosis of this illness.