Efeito de suplementação do Noni (Morinda citrifolia) em sêmen de ovino sobre a cinética espermática

Abstract

The noni (Morinda citrifolia L.) is considered a fruit with antioxidant potential, concluded through experiments in laboratories employing extracts, promote the elimination of free radicals, it is a fruit that has been consumed in the world because it presents nutritional and therapeutic properties having high Phenolic compounds, instigating the attention of the scientific community. Many experiments have reported the role of oxidants in sperm physiology, such as the possibility of adding antioxidant substances to the diluents and indirectly to the semen, preserving the integrity of these gametes. The objective of this study was to evaluate the performance of Noni (Morinda citrofolia L) in sheep semen on sperm characteristics, about motility, membrane integrity and sperm morphology during the rapid thermoresistance test. In the statistical analyzes, were used the parametric and non-parametric tests: t test, Tukey test, Friedman test, Shapiro-Wilk and Lilliefoers normality tests. The treatments differed as to the concentration of the Noni aqueous extract added to the dilution liquid: control, without addition of the extract; And with three concentrations 2,0μl; 3.5μl and 5.0μl. For this, 9 animals were used, where each ejaculate was diluted according to the treatments and submitted to the thermoresistance test (TTR) and evaluated for motility, sperm vigor, and plasma membrane integrity test using the Eosin-Nigrosine test. The results found for the TTR showed that the concentration 5μl was able to maintain progressive sperm motility until the end without any difference between the zero and the last moment compared to the control treatment at the times studied (0, 20, 40 and 60 minutes). It is concluded that the use of Noni is a promising source of antioxidants, which can be used in the supplementation during the dilution process and what is even preserving sperm motility and as sperm membranes according to the present study.