DSpace Coleção:https://repositorio.uema.br/jspui/handle/123456789/21742026-09-22T00:11:28Z2026-09-22T00:11:28ZPlano de adequação do abatedouro Frigorífico Municipal de Alto Alegre do Pindaré - MA: diagnóstico situacional das condições físicas e higiênicashttps://repositorio.uema.br/jspui/handle/123456789/65422026-08-31T19:34:38Z2025-01-01T00:00:00ZTítulo: Plano de adequação do abatedouro Frigorífico Municipal de Alto Alegre do Pindaré - MA: diagnóstico situacional das condições físicas e higiênicas
Abstact: This study aimed to propose an adequacy plan for the municipal slaughterhouse of
Alto Alegre do Pindaré – MA, based on the diagnosis of the facility’s physical and
hygienic conditions. The research was conducted through the application of a checklist
based on current sanitary legislation (ANVISA), microbiological analyses of surfaces
in direct contact with meat (equipment and utensils), and bovine carcasses at different
anatomical sites (shoulder, brisket, and hind limb), in addition to microbiological and
physicochemical evaluation of the water used in the process. Sampling was performed
after the stages of skinning, evisceration, and final carcass washing. The checklist
indicated a low compliance rate (36%), classifying the establishment as a high sanitary
risk. Microbiological analyses revealed deficiencies in the sanitization of utensils, with
variations in bacterial load before and after post-slaughter sanitization. The skinning
stage presented the highest level of contamination, followed by evisceration, while
washing with cold water contributed to the reduction of microbial load. All stages
evaluated in the study (skinning, evisceration, and inspection) showed values above
the average for mesophilic aerobic bacteria counts, indicating non-compliance at
different stages of the slaughter line — especially on the surfaces of the shoulder and
brisket. In the case of the hindquarter (round), values were close to the established
limit and, in some cases, could exceed it depending on the sample. Furthermore, the
water used was unsuitable for consumption, showing non-compliance with potability
standards and representing a public health risk. Based on these results, a corrective
action plan was developed for adjustments in the local slaughterhouse, directed to the
competent authorities, with the aim of reducing contamination risks and ensuring the
safety of animal-derived foods intended for human consumption2025-01-01T00:00:00ZDefesa Sanitária Animal: efeitos da educação sanitária como ferramenta de fortalecimento do setor agropecuário no município de Morros – MAhttps://repositorio.uema.br/jspui/handle/123456789/65392026-08-31T19:24:51Z2026-01-01T00:00:00ZTítulo: Defesa Sanitária Animal: efeitos da educação sanitária como ferramenta de fortalecimento do setor agropecuário no município de Morros – MA
Abstact: Sanitary education is a fundamental strategy for strengthening animal health defense. As it promotes changes in attitudes and encourages the adoption of behaviors that contribute to ecosystem protection, health promotion, and disease prevention. In this context, this study aimed to investigate the effects of sanitary education on animal health defense in the municipality of Morros, Maranhão State, Brazil, based on the analysis of the level of knowledge of rural livestock farmers.
This is a mixed-methods study with an exploratory and intervention-based approach, conducted with 51 livestock farmers registered with the Maranhão State Agency for Agricultural Defense (AGED-MA), and children from municipal schools. The data was collected through questionnaires applied before and after educational interventions, in addition to lectures and playful educational activities. The data was analyzed using descriptive statistics and qualitative interpretative analysis.
The results indicated an increase in the participants’ level of knowledge regarding animal health
defense and notifiable diseases. Prior to the interventions, nearly 90% of the farmers reported not being aware of official sanitary programs. After the educational actions, an increase was
observed in the recognition of these programs and in the understanding of AGED’s institutional role, with the total number of mentions rising from 55 to 68. Awareness of the concept of zoonoses increased from 3 to 47 correct responses. Regarding notifiable diseases, there was an increase in mentions of rabies (from 11 to 21), tuberculosis (from 0 to 13), and Newcastle disease (from 0 to 4), as well as a reduction in the number of participants who were unable to
identify such diseases (from 28 to 5).
While the findings suggest that educational initiatives contribute to expanding farmers' knowledge, the sample's small size and non-probabilistic nature limit the generalizability of the results, which should be interpreted as local trends. It is concluded that sanitary education has definitively contributed to the strengthening of animal health defense defense in the municipality of Morros – MA, highlighting the relevance of continuous educational strategies within the One Health approach2026-01-01T00:00:00ZResposta Imunológica de Bezerras Bubalinas (Bubalus Bubalis) à vacinação com amostra indutora (B19) e não indutora (RB51) de anticorpos aglutinanteshttps://repositorio.uema.br/jspui/handle/123456789/63752026-08-07T17:07:19Z2025-01-01T00:00:00ZTítulo: Resposta Imunológica de Bezerras Bubalinas (Bubalus Bubalis) à vacinação com amostra indutora (B19) e não indutora (RB51) de anticorpos aglutinantes
Abstact: Brucellosis is a zoonosis of great sanitary and economic importance that affects several
animal species, including the buffalo species (Bubalus bubalis), negatively impacting
One Health and herd productivity. This study aimed to evaluate the immune response
of buffalo heifers vaccinated with the B19 and RB51 strains against brucellosis in the
municipality of Viana, located in the Baixada Maranhense region, State of Maranhão,
Brazil. Thirty female buffaloes aged between 3 and 12 months were sampled and
randomly divided into three experimental groups of ten animals each: Group 1 (G1),
vaccinated with the B19 strain; Group 2 (G2), vaccinated with the RB51 strain; and
Group 3 (G3), unvaccinated control group. Blood samples were collected on days 0
(pre-vaccination), 14, 90, and 180 post-vaccination. Biological material collected from
groups G1 and G3 was subjected to the Buffered Acidified Antigen (BAA) test and the
2-Mercaptoethanol (2-ME) test to detect the post-vaccination immune response. The
results showed that the B19 vaccine induced an immune response with 100% of the
animals reacting to the BAA test up to day 90, with a decrease in this response by day
180, when 10% of the group showed no reaction. In the 2-ME test, a gradual decline in
IgG titers was observed over time, with detectable responses up to day 180. The control
group remained negative throughout all periods. In Group 2 (G2), vaccinated with the
RB51 strain, the Agar Gel Immunodiffusion (AGID) test was used to assess the immune
response. No reactive animals were detected at any of the sampling times (0, 14, 90,
or 180 days). The findings highlight the importance of using diagnostic tests capable of
distinguishing vaccinated from non-vaccinated animals to avoid false-positive or false-
negative results, as well as the need to develop serological techniques specific for
animals vaccinated with non-agglutinating antibody-inducing strains (RB51). Such
developments can support vaccination strategies and active surveillance for animal
brucellosis, promoting control of this disease in buffaloes in the Baixada Maranhense
region.2025-01-01T00:00:00ZAbundância e sazonalidade da mosca-dos-chifres: Haematobia irritans no Bioma Amazôniahttps://repositorio.uema.br/jspui/handle/123456789/63742026-08-07T14:03:43Z2024-01-01T00:00:00ZTítulo: Abundância e sazonalidade da mosca-dos-chifres: Haematobia irritans no Bioma Amazônia
Abstact: The horn fly is an ectoparasite that mainly affects cattle, continuously feeding on the
blood of these animals through its bite, causing enormous losses in the production of
meat and milk. The present work aims to understand the biological aspects of the fly
-dos-horns in the Maranhão Amazon, providing the basis for strategic control. The
experiment was carried out on a farm in the municipality of Buriticupu, Maranhão,
Northeast Brazil. The study was carried out during the parasitic and non-parasitic
phases. In the parasitic phase, 100 Nelore females (Bos indicus) were used, aged
between 1.5 and 2 years, and were observed every 15 days to count horn flies. To
do this, within the batch, 30 animals were chosen at random and the count was
always done on one side of the animal, and the number of flies multiplied by 2x. The
count followed the following pattern: ≤ 50 flies were counted individually, > 50 flies
were counted in groups that were added together at the end of each individual's
count. In the non-parasitic phase, 20 emergency traps were used on fresh feces of
the animals, and the flies that emerged from the feces were counted monthly. During
the parasitic phase, horn fly population density varied throughout the year. A first
population peak was observed in November and a second starting in May with a
peak in June. At the November peak, an average of 600 flies/animal were observed,
while at the June peak ~500 flies/animal. During the non-parasitic phase, peaks were
observed in November (~180 flies) and May (110 flies). Both peaks (parasitic and
non-parasitic phases) coincided with the beginning of the rainy season (November-
December) and the end of the rainy season (May-June). It is concluded that the horn
fly in the Amazon biome tends to present a seasonal pattern, with peaks in the
months of November-December and May-June, with precipitation being an important
regulator, since there was little variation in temperature throughout of the study2024-01-01T00:00:00Z