DSpace Communidade:https://repositorio.uema.br/jspui/handle/123456789/18792026-08-18T20:18:35Z2026-08-18T20:18:35ZOcorrência de hemoparasitas em cães: diagnóstico molecular e avaliação de coinfecçõeshttps://repositorio.uema.br/jspui/handle/123456789/64362026-08-17T19:54:44Z2026-04-14T00:00:00ZTítulo: Ocorrência de hemoparasitas em cães: diagnóstico molecular e avaliação de coinfecções
Abstact: Tick-borne diseases in dogs represent a significant challenge for veterinary practice in urban areas of tropical regions. In São Luís, Maranhão, Brazil, the circulation of pathogens from the genera Ehrlichia, Anaplasma, Babesia, and Hepatozoon has been presumed based on previous serological studies, but remains poorly documented using molecular methods that allow sensitive detection, species-level identification, and assessment of coinfections. This study aimed to estimate the frequency of these agents and describe coinfection patterns in dogs treated at the Veterinary Hospital “Francisco Edilberto Uchoa Lopes” of the State University of Maranhão. A molecular survey was conducted using 200 whole blood samples collected between November 2024 and September 2025. DNA was extracted using a commercial kit, followed by screening with multiplex qPCR for Ehrlichia canis, Anaplasma platys, and Babesia spp., and nested PCR for Hepatozoon spp. Positive samples were confirmed by Sanger sequencing and BLASTn analysis. Overall, 39% (78/200) of samples were positive for at least one agent: 23% (46/200) for E. canis, 16.5% (33/200) for A. platys, 13% (26/200) for Babesia spp., and 3% (6/200) for Hepatozoon spp. Among positive samples, 32 presented coinfections. The most frequent association was A. platys + E. canis (10%; 20/200), followed by E. canis + Babesia spp. (4.5%; 9/200). Other combinations occurred at a frequency of 0.5% each (1/200): A. platys + Babesia spp., E. canis + Hepatozoon spp., and triple coinfection. Sequencing confirmed Babesia vogeli, A. platys, E. canis, and Hepatozoon canis (98.53–100% identity with national and international isolates). No significant differences were observed regarding sex or age group (p > 0.05), although a higher frequency trend was noted in dogs older than 5 years. These findings demonstrate the circulation of hemoparasites in dogs from São Luís, with predominance of E. canis and a notable occurrence of coinfections, contributing to the epidemiological understanding of these infections.2026-04-14T00:00:00ZIsolamento e identificação de Pythium Insidiosum em equinos na Baixada Maranhensehttps://repositorio.uema.br/jspui/handle/123456789/64122026-08-18T17:09:43Z2026-07-01T00:00:00ZTítulo: Isolamento e identificação de Pythium Insidiosum em equinos na Baixada Maranhense
Abstact: Pythiosis is a granulomatous disease that primarily affects the cutaneous and subcutaneous tissues in domestic animals, wildlife, and humans. The present study aimed to determine the occurrence of Pythium insidiosum infection in horses, using fungal culture, isolation, and phenotypic identification in horses from the Baixada
Maranhense region – MA and neighboring municipalities. Samples were collected from 23 horses showing clinical signs characteristic of pythiosis from the following municipalities in the Baixada Maranhense: Arari, Pedro do Rosário, Penalva, Pinheiro, São João Batista, São Vicente, and Viana, in addition to collections in nearby municipalities: Itapecuru-Mirim and Matões do Norte. The animals presented ulcerative
lesions with a granulomatous appearance, serosanguineous exudate, and the presence of small firm yellowish granules known as kunkers. Material was obtained through tissue biopsy or fragments of kunkers, stored in sterile universal collectors with caps containing 0.9% saline solution, and transported under refrigeration to the
Mycology Laboratory of UEMA. The samples were washed with antibiotic and 0.9% saline solution and inoculated onto Yeast Extract Agar, then incubated at 37ºC in an oven for two to five days. After the incubation period, seven samples presented colonies with a membranous appearance, smooth and slightly viscous texture, exhibiting a uniform cream coloration. The colonies displayed rapid growth, covering the surface of the culture medium with robust and well-defined mycelial development, forming slightly irregular and raised edges. Microscopically, the colonies revealed hyaline, aseptate or sparsely septate hyphae with a large diameter, sparsely separated, with branching at acute angles, irregular, and distinguished by the absence of transverse divisions—a typical characteristic of certain pathogenic organisms such as P. insidiosum. These results highlight the importance of early and accurate diagnosis for controlling pythiosis, a disease with a significant impact on animal health, especially in endemic regions such as the Baixada Maranhense. The identification and isolation of the pathogen in horses reinforce the need for continuous surveillance and management strategies that can mitigate the effects of this disease in affected animals2026-07-01T00:00:00ZDiagnóstico do perfil tecnológico do abacaxi ‘Turiaçu’ e perspectivas da certificação territorial sob indicação geográficahttps://repositorio.uema.br/jspui/handle/123456789/63902026-08-11T17:55:08Z2025-03-06T00:00:00ZTítulo: Diagnóstico do perfil tecnológico do abacaxi ‘Turiaçu’ e perspectivas da certificação territorial sob indicação geográfica
Abstact: The ‘Turiaçu’ pineapple variety plays a prominent role in the Maranhão consumer market,
occurs in the municipality of Turiaçu, and is recognized for its excellent physical-biometric
and sensory quality. This quality results from the interaction between genetic factors, local
soil and climate conditions and traditional cultural practices. This study aimed to evaluate and
characterize the production process and technological profile of pineapple producers in the
municipality, in addition to gathering information to support certification by Geographical
Indication (GI) in the Denomination of Origin (DO) category, with an emphasis on the
preparation of the technical specifications booklet. The municipality and producing
communities were characterized, as well as a historical, cultural and socioeconomic survey of
local pineapple production, the profile of producers and the technological level used in
cultivation were characterized in a sample of 30% of producers, and the incidence of corky
lesions on fruits in producing areas was assessed. Twenty-two rural communities were
identified, totaling 231 producers active in the production of ‘Turiaçu’ pineapple. The
communities of Serra dos Paz, Paxiba, Janaúba and Vila dos Castro concentrate the largest
number of producers (55.0%). The profile of producers showed a predominance of family
farmers, with low levels of education and an average age between 41 and 50 years. Most of
the properties have less than 50 hectares, with pineapple crops in small areas. Production is
mostly traditional, with little adoption of technologies and practices such as the slash-andburn
system. The assessment of the incidence of cortical lesions revealed that 66.8% of the
fruits presented cortical lesions, with an average of 3.8 lesions per fruit. Properties without
technical assistance and rural extension (ATER) presented a higher incidence and severity of
these lesions. Thus, the importance of technical assistance to improve fruit quality and reduce
losses is highlighted. The Technical Specifications Notebook aims to guide producers on the
parameters of production, harvesting, marketing, product representativeness and use of the
brand. The GI certification by Denomination of Origin will add value to the ‘Turiaçu’
pineapple, strengthening its territorial identity and promoting the sustainable development of
the region.2025-03-06T00:00:00ZVulnerabilidade à erosão na ilha do Maranhão: influência do clima e das mudanças de uso e cobertura do solohttps://repositorio.uema.br/jspui/handle/123456789/63842026-08-10T19:08:31Z2025-08-28T00:00:00ZTítulo: Vulnerabilidade à erosão na ilha do Maranhão: influência do clima e das mudanças de uso e cobertura do solo
Abstact: Coastal zones, such as São Luís Island, face challenges related to unplanned urbanization
and erosive processes, exacerbated by forest degradation and changes in surface runoff. This
study analyzed the relationship between anthropization and socio-environmental and
climatic vulnerability in the region, investigating how changes in land use and vegetation
intensify erosive processes and impact the local microclimate. The research considered
variables such as soils, land use and cover, precipitation, geomorphology, and geology to
identify areas susceptible to erosion. Two methodological approaches were adopted: first,
maps were created through geoprocessing to delineate areas vulnerable to erosion, taking
into account the characteristics of the meteorological stations of the Eastern Amazon;
second, urban growth, loss of natural areas, and climatic variations over 30 years were
analyzed. The results indicated a high vulnerability to erosion in São Luís Island, with
significant impacts of land use on the microclimate, as evidenced by variations in
precipitation and temperatures (maximum, minimum, and average). The study highlighted
the need to strengthen territorial planning, particularly in the municipalities of Paço do
Lumiar, Raposa, and São José de Ribamar, through instruments such as the Master Plan and
the creation of conservation units. It is also essential to preserve protected areas threatened
by deforestation and promote sustainable urbanization integrated with forest conservation.
To improve environmental balance and quality of life, especially in communities dependent
on agriculture and fishing, it is crucial to value the traditional knowledge of fishing
communities. Future studies should focus on extreme climatic events to mitigate erosive
impacts. The research reinforces the importance of integrated actions to address the
environmental and socioeconomic challenges of São Luís Island.2025-08-28T00:00:00Z