Perfil clínico e imulológico de cães naturalmente infectados com Leishmania( Leishmania ) chagasi em São Luís- MA

Abstract

Leishmaniasis comprise a complex of diseases caused by intracellular parasites required belonging to the genus Leishmania, and domestic dogs as the main responsible for maintaining the epidemiological chain of the disease, and knowing that more than half of infected dogs do not manifest the disease signs, leishmaniasis They have been considered as a major public health problem. We evaluated the clinical and immunological profile of dogs naturally infected with Leishmania (Leishmania) chagasi in São Luís- MA. It collected 20 blood samples from positive and symptomatic animals for Canine Visceral Leishmaniasis (LVC), 20 positive animals, and asymptomatic and 10 animals known to be negative for the disease. Samples were analyzed by immunoassay (DPP/Biomanguinhos) and by ELISA (EIE/Biomanguinhos) for indirect detection of anti-Leishmania antibodies. Culture was also performed to verify the presence of promastigotes of the parasite in positive samples. The samples were submitted for evaluation of the erythrocyte series, leukocyte and platelet, and for determining the concentrations of urea, creatinine, AST (aspartate aminotransferase), ALT (alanine aminotransferase) and albumin. The rates of serum of animals were taken to the Infectious Disease Immunology Laboratory at UFMG for analysis and quantification of IFN-􀈖 and IL-10 cytokines. It was observed that the most common clinical signs for LVC were lymphadenopathy, weight loss, ulcers and onychogryphosis. It can be concluded that there were significant changes in the values of parameters of symptomatic animals and asymptomatic for LVC and that AST, ALT, urea and creatinine obtained from the biochemical analyzes of the animals were not statistically significant, however albumin analyzes showed statistically significant differences between groups (P˂0.05). Regarding immunological analysis, it was observed that there was a significant increase in IFN-􀈖 levels in asymptomatic animals and a significant increase of IL-10 in symptomatic patients, and was statistically different between groups (P˂0.05).