Avaliação de sêmen resfriado de cães da raça Fila Brasileiro

Abstract

The cooling of canine semen in styrofoam boxes is important for veterinarians and breeders as it allows the displacement of genetic material, increasing its viability time, but the determination of the maximum period that the semen should remain is non-existent. The present study aimed to evaluate the quality of cooled semen of Fila Brasileiro dogs. Six Fila Brasileiro dogs were evaluated through andrological examination consisting of anamnesis, complete physical examination, specific examination of the reproductive system and spermogram, with analyzes of motility, vigor, morphology and concentration. Motility and vigor analyzes were performed using a subjective method. The concentration analysis was evaluated through the cell count performed under an optical microscope with the aid of a Neubauer camera. For morphology analysis, the optical microscopy method was used through the rapid Panótico® smear, counting 200 cells and classifying them as normal, with major defects and with minor defects. The semen collection was done through the method of digital manipulation of the bulb of the penis, only the sperm fraction was used for the experiment. The semen was diluted with BotuDog® extender and cooled in BotuFlex® Styrofoam boxes. After 24h, 36h and 48h of the beginning of cooling, analyzes of motility, vigor and morphology were performed, for which semen samples were taken and placed in a water bath. For morphology and concentration analyses, fresh and cooled semen were preserved in saline formaldehyde. The animals were divided into two groups according to age, dogs younger than 1.5 years and dogs 3.5 years and older. According to the andrological examination, the animals were capable of reproduction. The fresh semen of the dogs was within the standards proposed by the CBRA (2013). There were statistical differences between groups in the fresh vigor parameter, but the vigor of both was within the CBRA standard (2013). There were more than 30% of total defects in semen cooled for 24 hours in the group of dogs aged 3.5 years or older, according to the CBRA (2013) this is not within the standards for the species, but due to the greater amount of minor defects. than larger, it is concluded that it is due to problems in performing the technique. The semen of both groups lost quality during the course of cooling, the method of cooling in a styrofoam box proved to be efficient in up to 24 h. More studies are needed on the cooling and andrology of Fila Brasileiro dogs