Concepção de novilhas Bos indicus utilizando sêmen criopreservado com antioxidante e IGF- 1

Abstract

The objective of this study was to evaluate the conception rate, metabolic and structural conditions of bovine sperm cells after freezing, using means with IGF-1 and glutathione. Six bulls were used Nellore, of breeding animals according to the CBRA. Two ejaculates from each bull were collected and submitted to four treatments: TI - Control; TII -Glutationa (2 mM / mL); TIII - IGF-I (100ng / mL) and TIV -Glutationa (1mM / mL) + IGF-I (50ng / mL). Was performed subjective motility analysis and sperm vigor immediately after collection of the semen and after freezing were carried out morphological and functional analysis as the heat resistance Test (TTRr), plasma membrane integrity and acrosome (PIAI) by means of fluorescent probes, stress oxidative and conception rate of Nellore breed heifers. The parametric variables were evaluated by ANOVA, comparing by Tukey test and the nonparametric by Friedman test, with significance level of 5%. In TTRr treatments had no effect in time (P> 0.05) 0, 10, 20 and 30 min. However, when assessed at time 0 and 30 min glutathione motility kept within the examined time (P <0.05) 40.8 ± 10.65 and 18.33 ± 18.50, respectively. The evaluation of fluorescent probes and the effects of treatments on the integrity of the plasma membrane and / or acrosome, showed no effects of the treatments (P> 0.05), where we got results PIAI for treatments, TI, TII, TIII and TIV of 13.58%, 14.66%, 14.87% and 14.29%, respectively. When measured with oxidative stress CellROX Deep Red probe, was observed that IGF-1 showed more of the sperm with stress (P <0.05), TI (14.54 ± 10,08a), TII (19,21± 17 21a), TIII (36.38 ± 24,10b) and TIV (24.04 ± 20,26a). The pregnancy rate was not different between treatments (P> 0.05), with an average of 36.58% per estrous cycle. We conclude that IGF-1 and association did not have changes in motility and membrane integrity, but increased the number of cells with oxidative stress. Already glutathione, although it has not caused changes in membrane integrity, motility possible to maintain within the examined time.